Hotspot

rps16

LSC · 4517-5611 · 1095 bp

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Pi 0.00703

MarkerSeek score 55.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1095
variable_sites: 35
pi: 0.00703
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAGGATCAGTCGTGGTCTT	TGATCCATTCCGGTAAGGGT	50.0	50.0	58.1	58.1	2288-2405 bp	0.933333	82.5
rps16_p2	TGAGGATCAGTCGTGGTCTT	TGCAGAGTCTTCCCCGATAA	50.0	50.0	58.1	58.1	1927-1962 bp	1.0	82.1
rps16_p3	GGAATTACACCGGACACTCC	TGATCCATTCCGGTAAGGGT	55.0	50.0	58.0	58.1	2119-2236 bp	0.8	77.5
rps16_p4	GGAATTACACCGGACACTCC	TGCAGAGTCTTCCCCGATAA	55.0	50.0	58.0	58.1	1760-1793 bp	0.866667	76.7
rps16_p5	GGAATTACACCGGACACTCC	TTGCAGAGTCTTCCCCGATA	55.0	50.0	58.0	58.1	1761-1794 bp	0.866667	76.7