Hotspot

ccsA-ndhD

SSC · 106516-106707 · 192 bp

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Pi 0.0159

MarkerSeek score 61.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 192
variable_sites: 15
pi: 0.0159
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCATTTGTAGCTTCGATAGGA	CTCTCGCTATCCGTTGACAG	42.9	55.0	55.9	57.9	352-360 bp	1.0	61.1
ccsA-ndhD_p2	GCATTTGTAGCTTCGATAGGA	TTGACAGGGTAGAGGCTCTC	42.9	55.0	55.9	58.1	339-347 bp	1.0	60.9
ccsA-ndhD_p3	GCATTTGTAGCTTCGATAGGA	GACAGGGTAGAGGCTCTCTT	42.9	55.0	55.9	57.8	337-345 bp	1.0	60.9
ccsA-ndhD_p4	GCATTTGTAGCTTCGATAGGA	TCTCTCGCTATCCGTTGACA	42.9	50.0	55.9	58.3	353-361 bp	1.0	60.0
ccsA-ndhD_p5	GCATTTGTAGCTTCGATAGGA	GTTGACAGGGTAGAGGCTCT	42.9	55.0	55.9	58.4	340-348 bp	1.0	58.5