Hotspot

ndhF-rpl32

SSC · 103798-104704 · 907 bp

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Pi 0.0119

MarkerSeek score 66.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 907
variable_sites: 51
pi: 0.0119
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	811-1009 bp	1.0	49.1
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	812-1010 bp	1.0	49.1
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	813-1011 bp	1.0	49.1
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	814-1012 bp	1.0	49.1
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATCGAAGTACGT	55.0	35.0	58.1	53.2	815-1013 bp	1.0	49.1