Hotspot

rpl33-rps18

LSC · 65485-65736 · 252 bp

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Pi 0.0226

MarkerSeek score 70.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 31
pi: 0.0226
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	AACGCCTACGAAAAGGTTGT	50.0	45.0	58.0	58.0	479-509 bp	1.0	86.2
rpl33-rps18_p2	AGAATCGCCACAATACACCC	ACGCCTACGAAAAGGTTGTT	50.0	45.0	58.0	58.0	478-508 bp	1.0	86.2
rpl33-rps18_p3	AGAATCGCCACAATACACCC	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.0	58.0	691-721 bp	1.0	84.7
rpl33-rps18_p4	AAGGAGGTTCATGGCAAAGG	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.1	58.0	810-840 bp	0.466667	61.5
rpl33-rps18_p5	GGAAAGGAGGTTCATGGCAA	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.1	58.0	813-843 bp	0.466667	61.4