Hotspot

psaJ-rpl33

LSC · 64885-65283 · 399 bp

Back to Triodia

Pi 0.00678

MarkerSeek score 43.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 16
pi: 0.00678
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	AACGCCTACGAAAAGGTTGT	45.0	45.0	57.9	58.0	1035-1097 bp	1.0	86.1
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	ACGCCTACGAAAAGGTTGTT	45.0	45.0	57.9	58.0	1034-1096 bp	1.0	86.1
psaJ-rpl33_p3	TTTGGGGCTTTAGCAGGTTT	GGGTGTATTGTGGCGATTCT	45.0	50.0	57.9	58.0	569-629 bp	1.0	85.6
psaJ-rpl33_p4	AAAACAAACGCGCTACCAAG	ACGCCTACGAAAAGGTTGTT	45.0	45.0	57.9	58.0	1512-1571 bp	1.0	84.5
psaJ-rpl33_p5	AAAACAAACGCGCTACCAAG	GGGTGTATTGTGGCGATTCT	45.0	50.0	57.9	58.0	1047-1104 bp	1.0	83.9