Hotspot

ycf4-cemA

LSC · 58387-58735 · 349 bp

Back to Triodia

Pi 0.00971

MarkerSeek score 62.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 16
pi: 0.00971
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.9	57.8	448-469 bp	1.0	83.3
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.1	57.8	450-471 bp	1.0	83.0
ycf4-cemA_p3	TTTCTTGCGCGTACCAATTG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.7	57.8	453-474 bp	1.0	81.3
ycf4-cemA_p4	CCTATTTCTTGCGCGTACCA	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.4	57.8	457-478 bp	1.0	81.2
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	477-498 bp	0.933333	79.4