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petN-trnC-GCA

LSC · 18120-18914 · 795 bp

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Pi 0.00771

MarkerSeek score 52.20

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 31
pi: 0.00771
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	843-850 bp	1.0	73.6
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	845-852 bp	1.0	73.1
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	CAGTTCAAATCTGGGTGCCG	50.0	55.0	58.2	59.5	844-851 bp	1.0	70.2
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	AGGGGACTGCAAATCCTTTA	50.0	45.0	58.2	56.4	868-875 bp	1.0	69.1
petN-trnC-GCA_p5	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTATCCCCAGT	50.0	42.9	58.2	57.2	861-868 bp	1.0	67.9