Hotspot

trnD-GUC-psbM

LSC · 16093-17120 · 1028 bp

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Pi 0.0093

MarkerSeek score 74.50

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 48
pi: 0.0093
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.6	56.7	1160-1208 bp	1.0	72.5
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	TCATTTTGGCTGGCTGTTTT	55.0	40.0	58.7	56.7	1193-1241 bp	1.0	71.6
trnD-GUC-psbM_p3	TCAATTGGTTAGAGCACCGC	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.6	56.5	1162-1210 bp	1.0	70.6
trnD-GUC-psbM_p4	TCAATTGGTTAGAGCACCGC	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.6	56.5	1161-1209 bp	1.0	70.6
trnD-GUC-psbM_p5	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.6	57.4	1160-1208 bp	1.0	70.4