Hotspot

trnY-GUA-trnD-GUC

LSC · 15677-16018 · 342 bp

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Pi 0.0118

MarkerSeek score 63.90

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 18
pi: 0.0118
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.0	58.6	453-536 bp	1.0	81.7
trnY-GUA-trnD-GUC_p2	GGGGACGGACTGTAAATTCG	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.4	58.6	420-503 bp	1.0	79.7
trnY-GUA-trnD-GUC_p3	GGGACGGACTGTAAATTCGT	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.6	58.6	419-502 bp	1.0	79.5
trnY-GUA-trnD-GUC_p4	AATTCTTCCTGGGTCGATGC	GGCGGTGCTCTAACCAATT	50.0	52.6	58.0	58.1	454-537 bp	1.0	77.0
trnY-GUA-trnD-GUC_p5	TGGGGACGGACTGTAAATTC	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.2	58.6	421-504 bp	1.0	76.3