Hotspot

trnG-UCC-trnM-CAU

LSC · 13846-14863 · 1018 bp

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Pi 0.00814
MarkerSeek score 66.20
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1018
variable_sites
38
pi
0.00814
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 424-1164 bp 1.0 47.6
trnG-UCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT GCCGTGGCATTACTCTACC 38.1 57.9 55.5 58.0 1142-1210 bp 0.933333 44.6
trnG-UCC-trnM-CAU_p3 AGAACGAATCACACTTTTACCAC GCCGTGGCATTACTCTACC 39.1 57.9 57.3 58.0 1145-1213 bp 0.933333 43.6
trnG-UCC-trnM-CAU_p4 GAACGAATCACACTTTTACCACT GCCGTGGCATTACTCTACC 39.1 57.9 57.3 58.0 1144-1212 bp 0.933333 43.6
trnG-UCC-trnM-CAU_p5 AACGAATCACACTTTTACCACT GCCGTGGCATTACTCTACC 36.4 57.9 56.2 58.0 1143-1211 bp 0.933333 42.7