Hotspot

rpl16

LSC · 76423-77857 · 1435 bp

Back to Triniochloa

Pi 0.00493

MarkerSeek score 62.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1435
variable_sites: 7
pi: 0.00493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GAACGAAAAACCTGGCGTTT	TTCCCGAAATCCCTGAAAAA	45.0	40.0	57.8	55.1	1557-1572 bp	1.0	56.6
rpl16_p2	GAACGAAAAACCTGGCGTTT	TTTCCCGAAATCCCTGAAAA	45.0	40.0	57.8	55.1	1558-1573 bp	1.0	56.6
rpl16_p3	GAACGAAAAACCTGGCGTTT	TTTTCCCGAAATCCCTGAAA	45.0	40.0	57.8	55.1	1559-1574 bp	1.0	56.6
rpl16_p4	GAACGAAAAACCTGGCGTTT	TTTTTCCCGAAATCCCTGAA	45.0	40.0	57.8	55.1	1560-1575 bp	1.0	56.6
rpl16_p5	GAACGAAAAACCTGGCGTTT	TTTCCCGAAATCCCTGAAAAA	45.0	38.1	57.8	56.0	1558-1573 bp	1.0	55.2