Hotspot

ndhF-trnL

SSC · 102224-103692 · 1469 bp

Back to Triniochloa

Pi 0.0171

MarkerSeek score 69.30

Primer available yes

Label ndhF-trnL

Feature Metadata

label_name: ndhF-trnL
feature_type: igs
region: SSC
length_bp: 1469
variable_sites: 25
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL_p1	GGAAGTGGGAGAAGAGGGAT	TTCGAACCGAGATGCTTGAG	55.0	50.0	58.1	58.0	1581-1585 bp	1.0	80.9
ndhF-trnL_p2	GGAAGTGGGAGAAGAGGGAT	GGATTCGAACCGAGATGCTT	55.0	50.0	58.1	58.1	1584-1588 bp	1.0	80.4
ndhF-trnL_p3	GGAAGTGGGAGAAGAGGGAT	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	1554-1558 bp	1.0	79.9
ndhF-trnL_p4	GGAAGTGGGAGAAGAGGGAT	AGCAGCGTGTCTACCAATTT	55.0	45.0	58.1	57.8	1547-1551 bp	1.0	79.5
ndhF-trnL_p5	GGAAGTGGGAGAAGAGGGAT	GCTTGAGCACTGCTTCCTAA	55.0	50.0	58.1	58.2	1568-1572 bp	1.0	79.4