Hotspot

rbcL-psaI

LSC · 55285-56249 · 965 bp

Back to Triniochloa

Pi 0.00832

MarkerSeek score 50.70

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 8
pi: 0.00832
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TCGAGTTCGAGCCAGTAGAT	AGAAGGTAAGTTGAAATCCGTCA	50.0	39.1	58.0	57.8	1024-1030 bp	1.0	54.8
rbcL-psaI_p2	CGCAGCTTGTGAAGTATGGA	AGAAGGTAAGTTGAAATCCGTCA	50.0	39.1	58.3	57.8	1056-1062 bp	1.0	52.9
rbcL-psaI_p3	GGCGATCAAATTCGAGTTCG	AGAAGGTAAGTTGAAATCCGTCA	50.0	39.1	57.6	57.8	1035-1041 bp	1.0	52.3
rbcL-psaI_p4	TGGAAGGCGATCAAATTCGA	AGAAGGTAAGTTGAAATCCGTCA	45.0	39.1	57.6	57.8	1040-1046 bp	1.0	52.0
rbcL-psaI_p5	TCGAGTTCGAGCCAGTAGAT	GAAGGTAAGTTGAAATCCGTCA	50.0	40.9	58.0	56.4	1023-1029 bp	1.0	51.7