Hotspot

ycf3-trnS

LSC · 43622-44220 · 599 bp

Back to Triniochloa

Pi 0.01

MarkerSeek score 64.50

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 6
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	786-792 bp	1.0	79.2
ycf3-trnS_p2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	736-742 bp	1.0	78.9
ycf3-trnS_p3	CATTTCCACGGGATCTAGGC	ACGGAAAGAGAGGGATTCGA	55.0	50.0	58.1	58.2	709-715 bp	1.0	78.5
ycf3-trnS_p4	CATTTCCACGGGATCTAGGC	TTCCAATGCTACGCCTTGAA	55.0	45.0	58.1	57.8	659-665 bp	1.0	78.3
ycf3-trnS_p5	TTTTTCCCCTGAGGTTGTCG	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.0	58.5	738-744 bp	1.0	76.1