Hotspot

trnR-rps14

LSC · 35710-36096 · 387 bp

Back to Triniochloa

Pi 0.011

MarkerSeek score 65.10

Primer available yes

Label trnR-rps14

Feature Metadata

label_name: trnR-rps14
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 4
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-rps14_p1	AGAAGACCTCTGTCCTATCCA	TATCCGGACACGTACTTCGA	47.6	50.0	56.9	58.0	471-494 bp	1.0	67.9
trnR-rps14_p2	AGGTTTAGAAGACCTCTGTCCT	TATCCGGACACGTACTTCGA	45.5	50.0	57.9	58.0	477-500 bp	1.0	67.2
trnR-rps14_p3	AGAAGACCTCTGTCCTATCCA	GCTATCCGGACACGTACTTC	47.6	55.0	56.9	57.9	473-496 bp	1.0	67.1
trnR-rps14_p4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	430-453 bp	1.0	66.8
trnR-rps14_p5	AGGTTTAGAAGACCTCTGTCCT	GCTATCCGGACACGTACTTC	45.5	55.0	57.9	57.9	479-502 bp	1.0	66.4