Hotspot

trnS-psbD

LSC · 7957-8943 · 987 bp

Back to Triniochloa

Pi 0.00715

MarkerSeek score 63.60

Primer available yes

Label trnS-psbD

Feature Metadata

label_name: trnS-psbD
feature_type: igs
region: LSC
length_bp: 987
variable_sites: 7
pi: 0.00715
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbD_p1	ATTAGCAATCCGCCGCTTTA	GACAAAAACGAAACGGTCCC	45.0	50.0	58.0	57.9	1120-1136 bp	1.0	79.9
trnS-psbD_p2	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	45.0	55.0	58.0	58.1	1141-1157 bp	1.0	79.9
trnS-psbD_p3	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1119-1135 bp	1.0	78.8
trnS-psbD_p4	ATTAGCAATCCGCCGCTTTA	CCGACAAAAACGAAACGGTC	45.0	50.0	58.0	58.3	1122-1138 bp	1.0	78.6
trnS-psbD_p5	ATTAGCAATCCGCCGCTTTA	CGACAAAAACGAAACGGTCC	45.0	50.0	58.0	58.3	1121-1137 bp	1.0	78.6