Hotspot

trnQ-psbI

LSC · 6707-7636 · 930 bp

Back to Triniochloa

Pi 0.0129

MarkerSeek score 57.60

Primer available yes

Label trnQ-psbI

Feature Metadata

label_name: trnQ-psbI
feature_type: igs
region: LSC
length_bp: 930
variable_sites: 12
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-psbI_p1	GGTTCGAACCTCCGAGTAAC	CCTGGGTCATTGGATAAGAA	55.0	45.0	58.0	54.4	1076-1089 bp	1.0	51.9
trnQ-psbI_p2	GGTTCGAACCTCCGAGTAAC	CCTGGGTCATTGGATAAGAATC	55.0	45.5	58.0	56.3	1076-1089 bp	1.0	51.0
trnQ-psbI_p3	GGTTCGAACCTCCGAGTAAC	CCTGGGTCATTGGATAAGAAT	55.0	42.9	58.0	55.0	1076-1089 bp	1.0	49.0
trnQ-psbI_p4	GTTCGAACCTCCGAGTAACG	CCTGGGTCATTGGATAAGAA	55.0	45.0	58.4	54.4	1075-1088 bp	1.0	48.9
trnQ-psbI_p5	GTTCGAACCTCCGAGTAACG	CCTGGGTCATTGGATAAGAATC	55.0	45.5	58.4	56.3	1075-1088 bp	1.0	47.9