Hotspot

rps16-trnQ

LSC · 5531-6634 · 1104 bp

Back to Triniochloa

Pi 0.00637

MarkerSeek score 62.80

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1104
variable_sites: 7
pi: 0.00637
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	TAAAACCTGAAATGGGGCGT	45.0	45.0	58.1	57.7	1237-1250 bp	1.0	77.3
rps16-trnQ_p2	TCATGTCCTTCAAGTCGCAC	TAAAACCTGAAATGGGGCGT	50.0	45.0	58.2	57.7	1248-1261 bp	1.0	76.8
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	CGTTACTCGGAGGTTCGAAC	45.0	55.0	58.1	58.4	1186-1199 bp	1.0	76.6
rps16-trnQ_p4	TCATGTCCTTCAAGTCGCAC	CGTTACTCGGAGGTTCGAAC	50.0	55.0	58.2	58.4	1197-1210 bp	1.0	76.1
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCAGCGGGTTTTGATC	45.0	50.0	58.1	58.5	1207-1220 bp	1.0	75.4