Hotspot

ycf1

SSC · 121557-127001 · 5445 bp

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Pi 0.0295

MarkerSeek score 56.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5445
variable_sites: 159
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCCATCACTGGGACATGT	AGCCCAGTTTACGAAGATTCT	45.0	42.9	56.7	57.0	218-227 bp	1.0	61.5
ycf1_p2	TTTTCCATCACTGGGACATGT	AGCCCAGTTTACGAAGATTCT	42.9	42.9	57.5	57.0	219-228 bp	1.0	59.5
ycf1_p3	TTTCCATCACTGGGACATGT	AGCCCAGTTTACGAAGATTCTT	45.0	40.9	56.7	57.7	218-227 bp	1.0	59.1
ycf1_p4	TCTTTTCCATCACTGGGACA	AGCCCAGTTTACGAAGATTCT	45.0	42.9	56.4	57.0	221-230 bp	1.0	58.5
ycf1_p5	TTTCCATCACTGGGACATGT	GCCCAGTTTACGAAGATTCT	45.0	45.0	56.7	55.5	217-226 bp	1.0	57.1