Hotspot

clpP1-psbB

LSC · 70762-71176 · 415 bp

Back to Trimezia

Pi 0.0594

MarkerSeek score 73.70

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 24
pi: 0.0594
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	GGGAAAGATACGCCCGAATT	CTCCCGAAATACTCCAACCG	50.0	55.0	58.0	58.1	1193 bp	0.5	59.2
clpP1-psbB_p2	GGGAAAGATACGCCCGAATT	CCAAAGCCAAAGCAAGCTAC	50.0	50.0	58.0	57.9	1407 bp	0.5	59.1
clpP1-psbB_p3	TTTTTGGGCGAAACAGAGGA	CTCCCGAAATACTCCAACCG	45.0	55.0	57.9	58.1	816 bp	0.5	59.0
clpP1-psbB_p4	GGGAAAGATACGCCCGAATT	CCCGCTAATATGCCCAATGT	50.0	50.0	58.0	58.1	1584 bp	0.5	59.0
clpP1-psbB_p5	TTTTTGGGCGAAACAGAGGA	CCAAAGCCAAAGCAAGCTAC	45.0	50.0	57.9	57.9	1030 bp	0.5	59.0