Hotspot

petA-psbJ

LSC · 62132-63261 · 1130 bp

Back to Trimezia

Pi 0.0484

MarkerSeek score 76.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 51
pi: 0.0484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	ATGTGGAGGAAAGTGAGGGA	50.0	50.0	58.0	58.0	1460-1507 bp	1.0	86.6
petA-psbJ_p2	CAGGGCCAGAACTTCTTGTT	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1764-1810 bp	1.0	85.9
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	ACGGACTAGCTGTACCTACC	50.0	55.0	58.0	58.0	1766-1812 bp	1.0	85.8
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1985-2031 bp	1.0	85.5
petA-psbJ_p5	CAGGGCCAGAACTTCTTGTT	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	1996-2042 bp	1.0	85.2