Hotspot

trnT-UGU-trnL-UAA

LSC · 45728-46518 · 791 bp

Back to Trimezia

Pi 0.0417
MarkerSeek score 72.90
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
791
variable_sites
30
pi
0.0417
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 CTAACCTCTGAGCTAAGCGG ACCAATTTCGCCATATCCCC 55.0 50.0 57.5 58.0 769-833 bp 1.0 83.0
trnT-UGU-trnL-UAA_p2 CTAACCTCTGAGCTAAGCGG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.5 58.1 777-841 bp 1.0 82.4
trnT-UGU-trnL-UAA_p3 CTAACCTCTGAGCTAAGCGG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.5 58.2 783-847 bp 1.0 81.0
trnT-UGU-trnL-UAA_p4 CTAACCTCTGAGCTAAGCGG TCAATCAAGTCCGTAGCGTC 55.0 50.0 57.5 57.7 790-854 bp 1.0 80.7
trnT-UGU-trnL-UAA_p5 CTAACCTCTGAGCTAAGCGG CAATCAAGTCCGTAGCGTCT 55.0 50.0 57.5 57.7 789-853 bp 1.0 80.7