Hotspot

psbC-trnS-UGA

LSC · 34694-34827 · 134 bp

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Pi 0.072
MarkerSeek score 76.10
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
134
variable_sites
9
pi
0.072
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 247-256 bp 1.0 84.3
psbC-trnS-UGA_p2 CTGTTCTTTCCATGACCCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 246-255 bp 1.0 84.3
psbC-trnS-UGA_p3 CCTGTTCTTTCCATGACCCC GGCTGAGTGGTTGATAGCTC 55.0 55.0 58.2 58.1 240-249 bp 1.0 84.2
psbC-trnS-UGA_p4 CTGTTCTTTCCATGACCCCC GGCTGAGTGGTTGATAGCTC 55.0 55.0 58.2 58.1 239-248 bp 1.0 84.2
psbC-trnS-UGA_p5 ATCGCGATTTGGAACCTGTT GAGAGATGGCTGAGTGGTTG 45.0 55.0 58.2 58.0 261-270 bp 1.0 83.8