Hotspot

psbI-trnS-GCU

LSC · 7585-7731 · 147 bp

Back to Trimezia

Pi 0.0752

MarkerSeek score 74.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 10
pi: 0.0752
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.1	58.2	180-194 bp	1.0	79.1
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	TTCGAATCCCTCTCTCTCCG	50.0	55.0	57.1	58.3	177-191 bp	1.0	78.3
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGAA	GGGTTCGAATCCCTCTCTCT	47.6	55.0	57.8	58.2	180-194 bp	1.0	76.7
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGAA	TTCGAATCCCTCTCTCTCCG	47.6	55.0	57.8	58.3	177-191 bp	1.0	76.0
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	GTTCGAATCCCTCTCTCTCC	50.0	55.0	57.1	56.8	178-192 bp	1.0	71.5