Hotspot

psbK-psbI

LSC · 7070-7473 · 404 bp

Back to Trimezia

Pi 0.034

MarkerSeek score 72.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 13
pi: 0.034
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTACGTCCCGGATCGTTAGA	50.0	50.0	57.9	58.0	521-526 bp	1.0	85.2
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	ACGTCCCGGATCGTTAGATA	50.0	50.0	57.9	57.7	519-524 bp	1.0	83.4
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TACGTCCCGGATCGTTAGAT	50.0	50.0	57.9	57.7	520-525 bp	1.0	83.4
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TTACGTCCCGGATCGTTAGA	45.0	50.0	58.3	58.0	518-523 bp	1.0	83.2
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TTACGTCCCGGATCGTTAGA	45.0	50.0	58.3	58.0	519-524 bp	1.0	83.2