Hotspot

trnK-UUU-rps16

LSC · 4284-4962 · 679 bp

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Pi 0.0387
MarkerSeek score 72.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
679
variable_sites
25
pi
0.0387
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCGCACTTAAAAGCCGAGTA TCAAGAAAGAACTTCGAGTT 45.0 35.0 57.3 52.4 717-744 bp 1.0 46.4
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG TCAAGAAAGAACTTCGAGTT 50.0 35.0 58.6 52.4 719-746 bp 1.0 46.3
trnK-UUU-rps16_p3 AGTCGCACTTAAAAGCCGAG TTCAAGAAAGAACTTCGAGT 50.0 35.0 58.6 52.4 720-747 bp 1.0 46.3
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG TTCAAGAAAGAACTTCGAGTT 50.0 33.3 58.6 53.3 720-747 bp 1.0 46.3
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG TTTCAAGAAAGAACTTCGAGT 50.0 33.3 58.6 53.3 721-748 bp 1.0 46.3