Hotspot

atpB

LSC · 53020-54516 · 1497 bp

Back to Trillium

Pi 0.305

MarkerSeek score 64.20

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 685
pi: 0.305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	TGCCTCTACTCGTGTCCTAG	TATATGGCGCAACCCAATCC	55.0	50.0	58.0	58.1	2380 bp	0.5	58.9
atpB_p2	CTACAGCTGTGGCAATAGGG	TATATGGCGCAACCCAATCC	55.0	50.0	58.0	58.1	2129 bp	0.5	58.9
atpB_p3	TGCCTCTACTCGTGTCCTAG	TGGCCGTTGAAACCGAAATA	55.0	45.0	58.0	58.1	2161 bp	0.5	58.8
atpB_p4	CTACAGCTGTGGCAATAGGG	TGGCCGTTGAAACCGAAATA	55.0	45.0	58.0	58.1	1910 bp	0.5	58.8
atpB_p5	TGCCTCTACTCGTGTCCTAG	GGTGACATAAGTCCCTCCCT	55.0	55.0	58.0	58.1	2579 bp	0.5	58.8