Hotspot

accD

LSC · 57468-58931 · 1464 bp

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Pi 0.109

MarkerSeek score 70.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1464
variable_sites: 239
pi: 0.109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATCCGATGGATCCTTGGGAT	ATGAGAATTGGTTCCGTCGG	50.0	50.0	57.9	58.0	2398 bp	0.5	59.4
accD_p2	ATCCGATGGATCCTTGGGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	2335 bp	0.5	59.4
accD_p3	ATCCGATGGATCCTTGGGAT	TGTGTTCGAAAAGAGCCACA	50.0	45.0	57.9	58.0	2489 bp	0.5	59.3
accD_p4	ATCCGATGGATCCTTGGGAT	GGGCACAAAAATGGAGGGTA	50.0	50.0	57.9	58.1	2301 bp	0.5	59.0
accD_p5	GGGCTCCTTCTACTCATCCT	ATGAGAATTGGTTCCGTCGG	55.0	50.0	57.9	58.0	2326 bp	0.5	59.0