Hotspot

ycf2-trnI-CAU

IRa · 152864-153023 · 160 bp

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Pi 0.192

MarkerSeek score 64.50

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 160
variable_sites: 46
pi: 0.192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	CCAAATTCGGGGGTCAAAGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	300-406 bp	1.0	91.3
ycf2-trnI-CAU_p2	CTCCAAATTCGGGGGTCAAA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	302-408 bp	1.0	91.3
ycf2-trnI-CAU_p3	TCCAAATTCGGGGGTCAAAG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	301-407 bp	1.0	91.3
ycf2-trnI-CAU_p4	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	889-929 bp	0.5	59.7
ycf2-trnI-CAU_p5	CGAACCCCGGTTAGATTCAG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.1	58.0	726-766 bp	0.5	59.4