Hotspot

atpB-rbcL

LSC · 54517-55210 · 694 bp

Back to Trillium

Pi 0.118

MarkerSeek score 74.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 123
pi: 0.118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	55.0	50.0	58.0	58.0	1653 bp	0.5	60.0
atpB-rbcL_p2	GCCGACTGGAACACTTAGAG	AGATCCTCCAGACGTAGAGC	55.0	55.0	58.0	58.0	1416 bp	0.5	59.9
atpB-rbcL_p3	GCCGACTGGAACACTTAGAG	ATCCTCCAGACGTAGAGCTC	55.0	55.0	58.0	58.0	1414 bp	0.5	59.9
atpB-rbcL_p4	GCCGACTGGAACACTTAGAG	GATCCTCCAGACGTAGAGCT	55.0	55.0	58.0	58.0	1415 bp	0.5	59.9
atpB-rbcL_p5	GCCGACTGGAACACTTAGAG	TCTCGATCTGGTAGCATCGT	55.0	50.0	58.0	58.0	1268 bp	0.5	59.9