Hotspot

ycf1

SSC · 118384-123726 · 5343 bp

Back to Trigonotis

Pi 0.00268

MarkerSeek score 54.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5343
variable_sites: 88
pi: 0.00268
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAATGGTATCCCAGGCTTC	AATGTGCACTCCCCACTTTT	55.0	45.0	57.7	57.9	215 bp	1.0	80.1
ycf1_p2	GGAATGGTATCCCAGGCTTC	ATGTGCACTCCCCACTTTTT	55.0	45.0	57.7	57.9	214 bp	1.0	80.1
ycf1_p3	GGAATGGTATCCCAGGCTTC	AAATGTGCACTCCCCACTTT	55.0	45.0	57.7	57.9	216 bp	1.0	80.0
ycf1_p4	GGAATGGTATCCCAGGCTTC	CTATACGTGCTCAAAGGCGT	55.0	50.0	57.7	58.1	265 bp	1.0	79.6
ycf1_p5	GGAATGGTATCCCAGGCTTC	TACGTGCTCAAAGGCGTAAA	55.0	45.0	57.7	57.8	262 bp	1.0	79.0