Hotspot

psbE-petL

LSC · 61639-62604 · 966 bp

Back to Trigonotis

Pi 0.00477

MarkerSeek score 54.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 26
pi: 0.00477
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1034-1069 bp	1.0	82.9
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1036-1071 bp	1.0	82.9
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GCCGCTAGTAGAAAACCGAA	55.0	50.0	58.2	58.0	1029-1064 bp	1.0	82.6
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AAGCCGCTAGTAGAAAACCG	55.0	50.0	58.2	58.0	1031-1066 bp	1.0	82.6
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.4	58.0	1027-1062 bp	1.0	81.4