Hotspot

rbcL-psaI

LSC · 55312-56621 · 1310 bp

Back to Trigonotis

Pi 0.005

MarkerSeek score 63.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1310
variable_sites: 33
pi: 0.005
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AGGCTACCAAATGGAGTCCT	GCCATTGAAATTGCCGGAAA	50.0	45.0	58.0	57.9	1046-1519 bp	1.0	84.9
rbcL-psaI_p2	GGCTACCAAATGGAGTCCTG	GCCATTGAAATTGCCGGAAA	55.0	45.0	58.0	57.9	1045-1518 bp	1.0	84.8
rbcL-psaI_p3	TTCGTGAGGCTACCAAATGG	GCCATTGAAATTGCCGGAAA	50.0	45.0	57.9	57.9	1052-1525 bp	1.0	84.3
rbcL-psaI_p4	GCTGCTTGTGAGGTATGGAA	GCCATTGAAATTGCCGGAAA	50.0	45.0	57.9	57.9	1017-1490 bp	1.0	84.3
rbcL-psaI_p5	AGGCTACCAAATGGAGTCCT	GAAGCCATTGAAATTGCCGG	50.0	50.0	58.0	58.4	1049-1522 bp	1.0	82.8