Hotspot

trnM-CAU-atpE

LSC · 51059-51232 · 174 bp

Back to Trigonotis

Pi 0.0211
MarkerSeek score 58.10
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
174
variable_sites
11
pi
0.0211
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TATTGCTTTCATACGGCGGG GGCAAATCTAGCTCTCCGTC 50.0 55.0 58.4 58.1 286-343 bp 1.0 81.9
trnM-CAU-atpE_p2 TGATGGGGCAGGTTTCATTT GGCAAATCTAGCTCTCCGTC 45.0 55.0 57.7 58.1 352-409 bp 1.0 81.5
trnM-CAU-atpE_p3 TTGATGGGGCAGGTTTCATT GGCAAATCTAGCTCTCCGTC 45.0 55.0 57.7 58.1 353-410 bp 1.0 81.4
trnM-CAU-atpE_p4 TGATGGGGCAGGTTTCATTT GAGGCAAATCTAGCTCTCCG 45.0 55.0 57.7 57.9 354-411 bp 1.0 81.4
trnM-CAU-atpE_p5 TTGATGGGGCAGGTTTCATT GAGGCAAATCTAGCTCTCCG 45.0 55.0 57.7 57.9 355-412 bp 1.0 81.4