Hotspot

trnV-UAC-trnM-CAU

LSC · 50793-50986 · 194 bp

Back to Trigonotis

Pi 0.000368

MarkerSeek score 26.20

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 1
pi: 0.000368
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	ATTGTATGACGGACTCCCCT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.8	58.4	312 bp	1.0	75.8
trnV-UAC-trnM-CAU_p2	ACCAACTGAGCTATAGCCCT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.8	58.4	257 bp	1.0	75.6
trnV-UAC-trnM-CAU_p3	CGGACTCCCCTGAAAAACAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.8	58.4	303 bp	1.0	75.4
trnV-UAC-trnM-CAU_p4	TATGACGGACTCCCCTGAAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.8	58.4	308 bp	1.0	75.0
trnV-UAC-trnM-CAU_p5	ATTGTATGACGGACTCCCCT	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.8	57.3	311 bp	1.0	73.8