Hotspot

rps4-trnT-UGU

LSC · 45286-45654 · 369 bp

Back to Trigonotis

Pi 0.00467

MarkerSeek score 39.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 7
pi: 0.00467
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 39.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGGTCCTCGATAACGTGACA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	440-464 bp	1.0	82.8
rps4-trnT-UGU_p2	AGGTCCTCGATAACGTGACA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.2	57.8	408-432 bp	1.0	82.0
rps4-trnT-UGU_p3	GAGGTCCTCGATAACGTGAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.5	57.9	441-465 bp	1.0	80.4
rps4-trnT-UGU_p4	AGGTCCTCGATAACGTGACA	ATTATGTAGGCCCGCTTAGC	50.0	50.0	58.2	57.5	459-483 bp	1.0	80.0
rps4-trnT-UGU_p5	AGGTCCTCGATAACGTGACA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.2	57.5	448-472 bp	1.0	79.9