Hotspot

ycf3-trnS-GGA

LSC · 43567-44391 · 825 bp

Back to Trigonotis

Pi 0.00661

MarkerSeek score 54.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 27
pi: 0.00661
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	AAGGTTCGGAAAGAGAGGGA	47.6	50.0	58.0	58.0	797-1031 bp	1.0	77.8
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	771-1005 bp	1.0	77.3
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TCGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	57.9	792-1026 bp	1.0	76.9
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	742-976 bp	1.0	76.6
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	AGGTTCGGAAAGAGAGGGAT	47.6	50.0	58.0	57.7	796-1030 bp	1.0	75.9