Hotspot

trnE-UUC-trnT-GGU

LSC · 30046-30692 · 647 bp

Back to Trigonotis

Pi 0.00647
MarkerSeek score 60.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
647
variable_sites
22
pi
0.00647
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 509-868 bp 1.0 85.0
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 507-866 bp 1.0 85.0
trnE-UUC-trnT-GGU_p3 TTCGTAGTACCACCCTACCC GAACCGATGACTTACGCCTT 55.0 50.0 57.9 58.0 565-924 bp 1.0 84.9
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 486-845 bp 1.0 84.6
trnE-UUC-trnT-GGU_p5 TTCGTAGTACCACCCTACCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.9 58.1 544-903 bp 1.0 84.5