Hotspot

trnR-UCU-atpA

LSC · 9692-9767 · 76 bp

Back to Trigonotis

Pi 0.0306

MarkerSeek score 59.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 76
variable_sites: 6
pi: 0.0306
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGGCGTCCATTGTCTAATG	GACATTTACTAAGGACGCGGA	50.0	47.6	58.0	57.8	225-243 bp	1.0	76.5
trnR-UCU-atpA_p2	TGGGCGTCCATTGTCTAATG	AGACATTTACTAAGGACGCGG	50.0	47.6	58.0	57.8	226-244 bp	1.0	76.5
trnR-UCU-atpA_p3	TGGGCGTCCATTGTCTAATG	ACATTTACTAAGGACGCGGA	50.0	45.0	58.0	56.7	224-242 bp	1.0	75.1
trnR-UCU-atpA_p4	TGGGCGTCCATTGTCTAATG	ACATTTACTAAGGACGCGGAA	50.0	42.9	58.0	57.4	224-242 bp	1.0	73.0
trnR-UCU-atpA_p5	TGGGCGTCCATTGTCTAATG	GACATTTACTAAGGACGCGG	50.0	50.0	58.0	56.4	225-243 bp	1.0	72.6