Hotspot

trnK-UUU-rps16

LSC · 4131-4921 · 791 bp

Back to Trigonotis

Pi 0.0128

MarkerSeek score 61.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 40
pi: 0.0128
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	GGTGCTCAACCTACAGGAAC	55.0	55.0	57.9	58.2	948-997 bp	1.0	85.1
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	887-936 bp	1.0	84.6
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	886-935 bp	1.0	84.6
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	896-945 bp	1.0	83.7
trnK-UUU-rps16_p5	TGGACGAATCCTTCCCTTCA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	939-988 bp	1.0	83.0