Hotspot

trnH-GUG-psbA

LSC · 82-345 · 264 bp

Back to Trigonotis

Pi 0.01
MarkerSeek score 56.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
264
variable_sites
14
pi
0.01
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CAACCGTGCTAACCTTGGTA 55.0 50.0 58.1 57.8 378-414 bp 1.0 84.4
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG ATCAACCGTGCTAACCTTGG 55.0 50.0 58.1 58.2 380-416 bp 1.0 84.4
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 392-428 bp 1.0 84.3
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 391-427 bp 1.0 84.3
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 393-429 bp 1.0 84.3