Hotspot

ycf1

SSC · 110473-115998 · 5526 bp

Back to Triglochin

Pi 0.0201
MarkerSeek score 57.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5526
variable_sites
110
pi
0.0201
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGGCTGTACGAAATGAAGTGT ACATCTAGAACAAAGGATTCTCT 42.9 34.8 57.6 54.8 280 bp 1.0 42.3
ycf1_p2 AGGCTGTACGAAATGAAGTGT ACATCTAGAACAAAGGATTCTCTT 42.9 33.3 57.6 55.5 280 bp 1.0 42.3
ycf1_p3 GGCTGTACGAAATGAAGTGTC ACATCTAGAACAAAGGATTCTCT 47.6 34.8 57.3 54.8 279 bp 1.0 42.3
ycf1_p4 AGGCTGTACGAAATGAAGTGT AGTACATCTAGAACAAAGGATTCTCT 42.9 34.6 57.6 57.4 283 bp 1.0 42.3
ycf1_p5 AGGCTGTACGAAATGAAGTGT ACATCTAGAACAAAGGATTCTC 42.9 36.4 57.6 53.3 280 bp 1.0 42.3