Hotspot

trnL-UAG-rpl32

SSC · 126147-126840 · 694 bp

Back to Triglochin

Pi 0.022
MarkerSeek score 51.50
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
694
variable_sites
2
pi
0.022
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.13
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC CCGCGGTTAAAGCTTTTTCC 50.0 50.0 58.0 58.3 806 bp 0.5 57.5
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA CCGCGGTTAAAGCTTTTTCC 50.0 50.0 58.0 58.3 807 bp 0.5 57.5
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA CCGCGGTTAAAGCTTTTTCC 50.0 50.0 57.9 58.3 816 bp 0.5 56.6
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC CCGCGGTTAAAGCTTTTTCC 55.0 50.0 57.8 58.3 815 bp 0.5 56.0
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC CCGCGGTTAAAGCTTTTTCC 55.0 50.0 57.8 58.3 814 bp 0.5 56.0