Hotspot

psbT-pbf1

LSC · 75100-75174 · 75 bp

Back to Triglochin

Pi 0.0533

MarkerSeek score 81.70

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 4
pi: 0.0533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	ACCACGATCGAACCTATGGA	TCGCCATCTCCATATCTGGT	50.0	50.0	58.2	58.0	305 bp	1.0	79.1
psbT-pbf1_p2	ACCACGATCGAACCTATGGA	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.2	58.1	311 bp	1.0	78.6
psbT-pbf1_p3	CGATCGAACCTATGGAAGCA	TCGCCATCTCCATATCTGGT	50.0	50.0	57.5	58.0	301 bp	1.0	76.9
psbT-pbf1_p4	ACGATCGAACCTATGGAAGC	TCGCCATCTCCATATCTGGT	50.0	50.0	57.5	58.0	302 bp	1.0	76.8
psbT-pbf1_p5	CGATCGAACCTATGGAAGCA	CCCTAGTCGCCATCTCCATA	50.0	55.0	57.5	58.1	307 bp	1.0	76.4