Hotspot

rbcL-psbE

LSC · 57644-58810 · 1167 bp

Back to Triglochin

Pi 0.0182

MarkerSeek score 70.20

Primer available yes

Label rbcL-psbE

Feature Metadata

label_name: rbcL-psbE
feature_type: igs
region: LSC
length_bp: 1167
variable_sites: 21
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psbE_p1	CGTGAAGCTTGCAAATGGAG	CAACCCGCAATGAATAGGGA	50.0	50.0	58.0	57.9	1354-1364 bp	1.0	81.3
rbcL-psbE_p2	CGTGAAGCTTGCAAATGGAG	ACTGACGAATAACCAACCCG	50.0	50.0	58.0	57.9	1367-1377 bp	1.0	81.0
rbcL-psbE_p3	GTTCAAACCGGTAGACACGA	CAACCCGCAATGAATAGGGA	50.0	50.0	57.9	57.9	1286-1296 bp	1.0	80.5
rbcL-psbE_p4	AGTTCAAACCGGTAGACACG	CAACCCGCAATGAATAGGGA	50.0	50.0	57.9	57.9	1287-1297 bp	1.0	80.5
rbcL-psbE_p5	CGTGAAGCTTGCAAATGGAG	ACGAATAACCAACCCGCAAT	50.0	45.0	58.0	58.2	1363-1373 bp	1.0	80.3