Hotspot

pafI-trnS-UCC

LSC · 45493-45960 · 468 bp

Back to Triglochin

Pi 0.0237
MarkerSeek score 65.10
Primer available yes
Label pafI-trnS-UCC

Feature Metadata

label_name
pafI-trnS-UCC
feature_type
igs
region
LSC
length_bp
468
variable_sites
11
pi
0.0237
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-UCC_p1 TCTCCTGAGGTTGTGGGAAT CCCTCGGTAAACAAAAGCCT 50.0 50.0 58.0 58.1 628-631 bp 1.0 80.7
pafI-trnS-UCC_p2 TCTCCTGAGGTTGTGGGAAT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.0 58.2 649-652 bp 1.0 80.2
pafI-trnS-UCC_p3 TCTCCTGAGGTTGTGGGAAT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.0 57.8 599-602 bp 1.0 80.0
pafI-trnS-UCC_p4 TTCTCCTGAGGTTGTGGGAA CCCTCGGTAAACAAAAGCCT 50.0 50.0 58.2 58.1 629-632 bp 1.0 79.5
pafI-trnS-UCC_p5 TTTCTCCTGAGGTTGTGGGA CCCTCGGTAAACAAAAGCCT 50.0 50.0 58.2 58.1 630-633 bp 1.0 79.5