Hotspot

atpH-atpI

LSC · 14638-15454 · 817 bp

Back to Triglochin

Pi 0.0202

MarkerSeek score 73.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 15
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	864-905 bp	1.0	80.9
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	920-961 bp	1.0	80.2
atpH-atpI_p3	AACGGAAGCGGCAGAAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	862-903 bp	1.0	79.7
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	865-906 bp	1.0	79.7
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.2	58.5	924-965 bp	1.0	79.6