Hotspot

tRNA-Leu-tRNA-Met

Genome · 118633-124924 · 6292 bp

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Pi 0.313

MarkerSeek score 75.60

Primer available yes

Label tRNA-Leu-tRNA-Met

Feature Metadata

label_name: tRNA-Leu-tRNA-Met
feature_type: igs
region: Genome
length_bp: 6292
variable_sites: 2653
pi: 0.313
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Leu-tRNA-Met_p1	CGTCATGTCATATAGGCCCG	AACCATGCAGTACGAGACAC	55.0	50.0	58.0	57.9	728 bp	0.333333	52.7
tRNA-Leu-tRNA-Met_p2	CGTCATGTCATATAGGCCCG	GGGCGTTTTTCGAATAAGCC	55.0	50.0	58.0	58.1	686 bp	0.333333	52.5
tRNA-Leu-tRNA-Met_p3	CGTCATGTCATATAGGCCCG	TGGAGTGACGTTGCTTCTTC	55.0	50.0	58.0	58.1	1067 bp	0.333333	52.2
tRNA-Leu-tRNA-Met_p4	CGTCATGTCATATAGGCCCG	AATTCATTTGGGACCCCGAG	55.0	50.0	58.0	57.9	666 bp	0.333333	52.2
tRNA-Leu-tRNA-Met_p5	CGTCATGTCATATAGGCCCG	CAATTCATTTGGGACCCCGA	55.0	50.0	58.0	58.2	667 bp	0.333333	52.1