Hotspot

tRNA-Ile-tRNA-Ile

Genome · 110041-110712 · 672 bp

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Pi 0.31

MarkerSeek score 81.00

Primer available yes

Label tRNA-Ile-tRNA-Ile

Feature Metadata

label_name: tRNA-Ile-tRNA-Ile
feature_type: igs
region: Genome
length_bp: 672
variable_sites: 274
pi: 0.31
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ile-tRNA-Ile_p1	ACGTTTTTGACAGCCCATCT	GAGGCAAAAAGGGGGAAAGA	45.0	50.0	58.0	58.0	1092 bp	0.333333	53.1
tRNA-Ile-tRNA-Ile_p2	ACGTTTTTGACAGCCCATCT	GGCAAAAAGGGGGAAAGAGA	45.0	50.0	58.0	58.0	1090 bp	0.333333	53.1
tRNA-Ile-tRNA-Ile_p3	ACGTTTTTGACAGCCCATCT	AGGCAAAAAGGGGGAAAGAG	45.0	50.0	58.0	58.0	1091 bp	0.333333	53.1
tRNA-Ile-tRNA-Ile_p4	AAAGTGAGCCACCAGTTCAG	GAGGCAAAAAGGGGGAAAGA	50.0	50.0	58.0	58.0	1015 bp	0.333333	53.0
tRNA-Ile-tRNA-Ile_p5	AAAGTGAGCCACCAGTTCAG	GGCAAAAAGGGGGAAAGAGA	50.0	50.0	58.0	58.0	1013 bp	0.333333	53.0