Hotspot

tRNA-Ser-tRNA-Gln

Genome · 79744-81240 · 1497 bp

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Pi 0.315

MarkerSeek score 80.50

Primer available yes

Label tRNA-Ser-tRNA-Gln

Feature Metadata

label_name: tRNA-Ser-tRNA-Gln
feature_type: igs
region: Genome
length_bp: 1497
variable_sites: 612
pi: 0.315
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Gln_p1	TGGACTAAAGCGTCGGATTG	CACGTTGCTTTTTACCGCAT	50.0	45.0	58.0	58.0	1904 bp	0.333333	52.9
tRNA-Ser-tRNA-Gln_p2	GTGGACTAAAGCGTCGGATT	CACGTTGCTTTTTACCGCAT	50.0	45.0	58.0	58.0	1905 bp	0.333333	52.9
tRNA-Ser-tRNA-Gln_p3	GAATTGGGGAGAGATGGCTG	CACGTTGCTTTTTACCGCAT	55.0	45.0	58.0	58.0	1926 bp	0.333333	52.8
tRNA-Ser-tRNA-Gln_p4	GAAAAGGGGGCGAATCTCAT	CACGTTGCTTTTTACCGCAT	50.0	45.0	57.9	58.0	2488 bp	0.333333	52.5
tRNA-Ser-tRNA-Gln_p5	AAGAGCCATGCCAGTACCTA	CACGTTGCTTTTTACCGCAT	50.0	45.0	58.1	58.0	2202 bp	0.333333	52.0